Thanks for the knowledge share and reply. The room was cleaned to start, brand new supplies, aka trays and pots, except ones priorly used which were all carefully sterilized before being put back into use. Initial plant matter was only tubers which had been washed months prior. I would go to thinking they were in the soil (fresh bales of pro-mix) as unhatched eggs, except there is no ant problem in the 1020 50-cell trays being used for cuttings - same soil. Plus 2 times seeing a trail of ants on the baseboard with the trail starting at electrical outlet in the baseboard and 1x around the window framing. I wouldnt think I would have had any aphids finding their way in under the above circumstance, right? Or no? Bobby just went out and sprayed around the foundation outside again. He says there is no indication out there of ants, aka no detectable ant hills nor any sign of ants crawling on the siding.
So since I wrote my initial post, I've started sprinkling the DE into a new tray, picking each pot up to see if anything is crawling around the outside or bottom of the pot, placing it down into the fresh DE tray. I found two ants that were in a tray that I'd missed in my inspection before sprinkling the DE into the tray, and lol, took pleasure in watching them die - roughly five minutes. All of this is painstaking procedure to solve this problem when I really need to be outside attending to all of THOSE pre-planting chores. Soooo very frustrated here!!! Rant over... but if anyone can shed further light on this problem, please by all means, share!